We examined the vascularity of mammary Paget disease histologically to confirm the increased blood\nflow observed previously by clinical imaging. The relationships among blood vessel density (BVD), histopathological\nparameters of blood flow in the nipple, and the expression of angiogenic factors such as basic fibroblast growth\nfactor (bFGF) and vascular endothelial growth factor A (VEGFA) were examined.\nMethods: We calculated the average CD34-positive BVD and podoplanin (D2-40)-positive lymphatic vessel density\n(LVD) and the proportion of proliferating of endothelial cells in 14 Paget disease, 3 dermatitis biopsy, and 14 agematched\ncontrol cases. As a parameter related to blood flow in the nipple, the total CD34-positive blood vessel\nlumen area relative to the entire nipple area was measured in each Paget disease and control case using an\nautomated image analysis system. Immunohistochemical expression of bFGF and VEGFA in Paget cells was also\nexamined..............
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